Journal: PLOS ONE

Article Title: A systematic review of non-coding RNA genes with differential expression profiles associated with autism spectrum disorders

doi: 10.1371/journal.pone.0287131

Figure Lengend Snippet: Studies examining ncRNA gene expression in ASD using tissue samples and laboratory methodologies that could be feasibly implemented into clinical practice (N = 33).

Article Snippet: 13:2 , 4.3 , Not stated: Sex and age matched , , 5 miRNA genes examined by qPCR on CFX96 BioRad , miR-16-5p.

Techniques: Gene Expression, Biomarker Discovery, Control, RNA Extraction, Gene Assay, Microarray, TLDA Assay, Sample Prep, Standard Deviation, SYBR Green Assay, Sequencing, Expressing, Isolation, Reverse Transcription, Clinical Proteomics, TaqMan microRNA Assay, Multiplex Assay, Construct, Fluorescence

Journal: Diabetologia

Article Title: Role of microRNAs in the age-associated decline of pancreatic beta cell function in rat islets

doi: 10.1007/s00125-015-3783-5

Figure Lengend Snippet: Islet miRNA expression in rats of different ages. miRNA expression was measured by qPCR. Results are presented as fold change vs the level in islets of 3-month-old animals and represent means ± SD; n = 5. * p < 0.05 vs 3-month-old mice by ANOVA and Dunnett’s post hoc test. Mo, month

Article Snippet: MiRNA expression was assessed using the miRCURY LNA Universal RT miRNA PCR kit (Exiqon, Vedbaek, Denmark).

Techniques: Expressing

Journal: Diabetologia

Article Title: Role of microRNAs in the age-associated decline of pancreatic beta cell function in rat islets

doi: 10.1007/s00125-015-3783-5

Figure Lengend Snippet: Age-associated changes in miR-34a expression in human islets and in other rat organs. RNA was isolated from the islets of human donors of various ages ( a ) and from the brain ( b ) and liver ( c ) of rats aged 3, 12 and 23 months. ( a ) The miR-34a level was positively correlated with the age of human islet donors. miRNA expression was measured using TaqMan Low Density Array plates (Life Technologies, Zug, Switzerland) and a global mean normalisation strategy to control for RNA input. Relative miR-34a levels were calculated using the mean ΔCt of all 11 samples and log 2 transformed for normality. miR-34a expression in brain ( b ) and liver ( c ) of rats. The data are expressed as fold change vs the level of expression in 3-month-old rats. ( b , c ) Data represent means ± SD * p < 0.05 vs 3-month-old rats by ANOVA and Dunnett’s post hoc test. Mo, month

Article Snippet: MiRNA expression was assessed using the miRCURY LNA Universal RT miRNA PCR kit (Exiqon, Vedbaek, Denmark).

Techniques: Expressing, Isolation, TLDA Assay, Control, Transformation Assay

Journal: Diabetologia

Article Title: Role of microRNAs in the age-associated decline of pancreatic beta cell function in rat islets

doi: 10.1007/s00125-015-3783-5

Figure Lengend Snippet: Impact of changes in miRNA expression on insulin secretion and content. Dissociated rat islet cells were transfected with oligonucleotides leading to overexpression ( a – c ) or downregulation ( d , e ) of the indicated miRNAs. Insulin secretion ( a – e ) and insulin content ( f , g ) in response to 2 mmol/l (black bars) or 20 mmol/l (white bars) glucose was measured 48 h post transfection. Insulin release is expressed as fold change vs 2 mmol/l glucose in the control (ctrl) condition. Data represent means ± SD

Article Snippet: MiRNA expression was assessed using the miRCURY LNA Universal RT miRNA PCR kit (Exiqon, Vedbaek, Denmark).

Techniques: Expressing, Transfection, Over Expression, Control

Journal: Diabetologia

Article Title: Role of microRNAs in the age-associated decline of pancreatic beta cell function in rat islets

doi: 10.1007/s00125-015-3783-5

Figure Lengend Snippet: Impact of changes in miRNA expression on apoptosis. Dispersed rat ( a , c , e ) and human ( b , d , f ) islet cells were transfected with oligonucleotides leading to overexpression ( a , b , e , f ) or downregulation ( c , d ) of the indicated miRNAs. Apoptosis was assessed by scoring the cells displaying pycnotic nuclei. Incubation of cells transfected with control (ctrl) oligonucleotides during 24 h with a mix of proinflammatory cytokines (10 ng/ml TNF-α, 0.1 ng/ml IL-1β and 30 ng/ml IFNγ; cyt) was used as a positive control for apoptosis. Results are the means ± SD of three or four independent experiments. * p < 0.05 (vs control for a , b ) by ANOVA and Dunnett’s post hoc test

Article Snippet: MiRNA expression was assessed using the miRCURY LNA Universal RT miRNA PCR kit (Exiqon, Vedbaek, Denmark).

Techniques: Expressing, Transfection, Over Expression, Incubation, Control, Positive Control

Journal: Diabetologia

Article Title: Role of microRNAs in the age-associated decline of pancreatic beta cell function in rat islets

doi: 10.1007/s00125-015-3783-5

Figure Lengend Snippet: Impact of changes in miRNA expression on beta cell proliferation. Rat islet cells were transfected with oligonucleotides leading to overexpression ( a , b , e , f ) or downregulation ( c , d , g ) of the indicated miRNAs and treated with exendin-4 (Ex-4; 100 nmol/l) or PDGF-AA (50 ng/ml) (black bars). Beta cell proliferation was assessed 48 h later by anti-Ki67 and anti-insulin staining. The results represent the means ± SD of three to six independent experiments. * p < 0.05 by ANOVA and Dunnett’s post hoc test. Ctrl, control

Article Snippet: MiRNA expression was assessed using the miRCURY LNA Universal RT miRNA PCR kit (Exiqon, Vedbaek, Denmark).

Techniques: Expressing, Transfection, Over Expression, Staining, Control

Journal: Cell Reports Medicine

Article Title: Discovery and Verification of Extracellular miRNA Biomarkers for Non-invasive Prediction of Pre-eclampsia in Asymptomatic Women

doi: 10.1016/j.xcrm.2020.100013

Figure Lengend Snippet: Previously Published Studies Reporting Extracellular miRNA Biomarkers for Prediction or Diagnosis of Pre-eclampsia

Article Snippet: Martinez-Fierro et al. Mexico , serum , miRNeasy Mini Kit (QIAGEN , TaqMan Low Density Array Human MicroRNA Card Set v2.0, focused on 51 C19MC miRNAs (Applied Biosystems) , 34 (16 PE, 18 control) , 12, 16, and/or 20 weeks, asymptomatic, longitudinal , –,–,–, p < 0.05 , hsa-miR-520c-3p (16 weeks); hsa-miR-512-3p, 518f-3p, 520d-3p (20 weeks) , .

Techniques: Biomarker Discovery, Isolation, Control, Clinical Proteomics, Microarray, TLDA Assay, Extraction

Journal: Cell Reports Medicine

Article Title: Discovery and Verification of Extracellular miRNA Biomarkers for Non-invasive Prediction of Pre-eclampsia in Asymptomatic Women

doi: 10.1016/j.xcrm.2020.100013

Figure Lengend Snippet:

Article Snippet: Martinez-Fierro et al. Mexico , serum , miRNeasy Mini Kit (QIAGEN , TaqMan Low Density Array Human MicroRNA Card Set v2.0, focused on 51 C19MC miRNAs (Applied Biosystems) , 34 (16 PE, 18 control) , 12, 16, and/or 20 weeks, asymptomatic, longitudinal , –,–,–, p < 0.05 , hsa-miR-520c-3p (16 weeks); hsa-miR-512-3p, 518f-3p, 520d-3p (20 weeks) , .

Techniques: Isolation, Picogreen Assay, Multiplex Assay, Software